# Biopanning

Analyzing NGS or Sanger sequences from biopanning and phage display experiments on PipeBio

## Identifying enriched sequences from biopanning

### Steps for finding enriched clones between multiple biopanning rounds

#### Annotation and quality control

- PipeBio’s annotation engine detects sequences with frameshifts, stop codons or missing regions
- You can create summary reports of the imported and analyzed NGS samples
- You can quickly filter the sequences in the Status dashboard and discard sequences with sequencing errors from subsequent analysis

#### Cluster and compare multiple samples

- You can cluster across multiple regions and determine the amino acid identity for cluster sequence similarity
- Use charts to see the largest clusters in the samples
- Easily identify which sequence clusters are most enriched across biopanning rounds

#### Differentially enriched antibodies

- Easily identify which antibody sequence clusters are most enriched across biopanning rounds on the Volcano Plot
- PipeBio calculates the normalized fold change between panning rounds and allows sample comparison across multiple rounds to show statistically significant clonal enrichment (p-value threshold)
- Visualize sample overlap and filter by clicking on interactive charts