# Antibody hit expansion

Analyzing NGS or Sanger antibody sequences from biopanning and phage, yeast or mammalian display experiments on PipeBio

## Identifying clonal families and similar antibodies

### Cluster around Sanger sequences and pick developable antibodies from NGS repertoire sequencing

#### Clustering around characterized hit antibody

- You can seed individual clusters from original hit antibodies that have shown target-specificity, in order to find closely related sequences with similar binding properties
- Use your characterized antibody clones as a centroid sequence and cluster together with NGS repertoire sequences
- Find related, developable sequences with less liabilities from the NGS repertoire with clustering and phylogenetic analysis