# Sanger sequence analysis

Tools for analyzing Sanger sequences on PipeBio

## How to analyze Sanger sequences

### Trim, assemble forward and reverse read chromatograms, detect heterozygous peaks, annotate, edit and align sequences

#### Sanger sequence quality control, assembly, variant calling

- Detect double peaks (heterozygous base calls) and edit nucleotide bases
- Assemble Sanger sequences sequenced with forward and reverse primers
- Detect SNPs from cloning or synthethization
- Compares parent with antibody clone by  multiple sequence alignment to reference

#### Automate Sanger sequence analysis

- Import .ab1 files
- Assemble chromatograms of your forward and reverse read Sanger sequences or detect double peaks
- Annotate Sanger sequences (IgG, VHH, scFv)
- Pair heavy and light chains of IgGs
- Align Sanger sequences, visualize clonal families and phylogeny
- Add functional assay data for binding affinity, antigen specificity and more
- Overlay sequences with metadata and hit-pick for DNA synthesis