Biopanning

Biopanning

Analyzing NGS or Sanger sequences from biopanning and phage display experiments on PipeBio

Identifying enriched sequences from biopanning

Steps for finding enriched clones between multiple biopanning rounds

Annotation and quality control

  • PipeBio’s annotation engine detects sequences with frameshifts, stop codons or missing regions
  • You can create summary reports of the imported and analyzed NGS samples
  • You can quickly filter the sequences in the Status dashboard and discard sequences with sequencing errors from subsequent analysis

Cluster and compare multiple samples

  • You can cluster across multiple regions and determine the amino acid identity for cluster sequence similarity
  • Use charts to see the largest clusters in the samples
  • Easily identify which sequence clusters are most enriched across biopanning rounds

Differentially enriched antibodies

  • Easily identify which antibody sequence clusters are most enriched across biopanning rounds on the Volcano Plot
  • PipeBio calculates the normalized fold change between panning rounds and allows sample comparison across multiple rounds to show statistically significant clonal enrichment (p-value threshold)
  • Visualize sample overlap and filter by clicking on interactive charts
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