Identifying liabilities

Identifying liabilities

You can define and identify sequence-based liabilities and automatically flag sequence motifs when annotating sequences on PipeBio

Liability detection

Accurately annotate, define and characterize regions and liabilities of antibody sequences to de-risk antibody development

Labeling sequences with liabilities

  • PipeBio's annotation tool can detect a wide range of liabilities in sequences that can affect the binding of the antibody with the relevant antigen, aggregation etc.
  • Validations include post-translational modifications (PTMs), quality validations and structural verifications
  • Validations are specified in the scaffold (left in image) with designated liability scores
  • The image shows deamidation and glycosylation sites in the CDR-H3 region

Annotate and detect sequence errors

  • PipeBio’s annotation engine is able to efficiently detect and label CDRs and framework regions in antibody sequences from both the heavy and light (kappa and lambda) chains
  • The variable (V), joining (J), diversity (D) and constant C gene segments are also identified thanks to our manually-curated germline databases
  • You can create custom germline databases to identify and annotate additional regions, such as linkers, tags, loops and more

Post-translational modifications (PTMs) in antibody development

The importance of accurate annotation of antibody sequences

Post-translational modifications cause additional sequence variation and alter the antibody binding properties. This might be troublesome when developing antibodies designed against a specific target.

Inaccurate annotation of sequences or detection of liabilities and identification of mutations will lead to decreased efficacy of promising lead candidates. Efficient and exact annotation tools are therfore needed to ensure the developability[1].

PTMs include the addition of functional groups in the amino acid chain, the modification of existing amino acids in the sequence or the cleavage of bonds, among others [3, 4].

Which liabilities can PipeBio detect?

Below is a list of liabilities detected by the default IgG scaffold on PipeBio:

  • Asparagine deamidation
  • Aspartate isomerization
  • N-linked glycosylation
  • Lysine glycation
  • Methionine oxidation
  • Tryptophan oxidation
  • Aspartic acid – Proline cleavage
  • Hydrolysis
  • Any custom motifs can be configured
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References

  • Raybould, M., & Deane, C. M. (2022). The Therapeutic Antibody Profiler for Computational Developability Assessment. Methods in molecular biology (Clifton, N.J.), 2313, 115–125. https://doi.org/10.1007/978-1-0716-1450-1_5
  • Ramazi, S. & Zahiri, J. (2021). Post-translational modifications in proteins: resources, tools and prediction methods. Database, Volume 2021, baab012. https://doi.org/10.1093/database/baab012
  • Lu, X., Nobrega, R. P., Lynaugh, H., Jain, T., Barlow, K., Boland, T., Sivasubramanian, A., Vásquez, M., & Xu, Y. (2019). Deamidation and isomerization liability analysis of 131 clinical-stage antibodies. mAbs, 11(1), 45–57. https://doi.org/10.1080/19420862.2018.1548233
  • Hattori, T., & Koide, S. (2018). Next-generation antibodies for post-translational modifications. Current opinion in structural biology, 51, 141–148. https://doi.org/10.1016/j.sbi.2018.04.006